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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC57A10.07
Gene ID 13/B02
Gene synonyms/obsolete
Gene product conserved hypothetical; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 936
ORF length (spliced)
Entry clone length 936
No. of intron 0
Sequence status Finished
Sequence results 398T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC57A10.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTAAATAAACGCTTAAC
Rev primer name SPAC57A10.07.Rv
Rev primer SEQ AGAAAGCTGGGTACCAAGGCAGTTTTCCATTA
Amino acid length 311
Molecular weight 35.1
Isoelectric point (calc.) 8.9
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LYASLILLCI/LKNLTHLII
Localization (YFP) cytosol=nucleus; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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