Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC9B6.07
Gene ID 13/B03
Gene synonyms/obsolete
Gene product involved in rRNA processing
Entry clone Cloned
ORF length (unspliced) 936
ORF length (spliced) 654
Entry clone length 936
No. of intron 3
Sequence status Finished
Sequence results 11C:T / 738T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC9B6.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGGCTCCGTCGCCGTT
Rev primer name SPBC9B6.07.Rv
Rev primer SEQ AGAAAGCTGGGTATTGTGAATCCTTTGTATAT
Amino acid length 217
Molecular weight 25.7
Isoelectric point (calc.) 9.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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