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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC222.11
Gene ID 13/B07
Gene synonyms/obsolete
Gene product coproporphyrinogen III oxidase
Entry clone Cloned
ORF length (unspliced) 939
ORF length (spliced)
Entry clone length 939
No. of intron 0
Sequence status Finished
Sequence results 190G:A / 460A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC222.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGACATTACCGTTGA
Rev primer name SPAC222.11.Rv
Rev primer SEQ AGAAAGCTGGGTAGCACCATTCAATAGGGGTA
Amino acid length 312
Molecular weight 36.2
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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