Gene name |
SPBC16E9.07 |
Gene ID |
13/B12 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan |
Entry clone |
Cloned# |
ORF length (unspliced) |
942 |
ORF length (spliced) |
|
Entry clone length |
942 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC16E9.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTATACAACTAAACGCTT |
Rev primer name |
SPBC16E9.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAAGGCCAAAATTGGTGCG |
Amino acid length |
313 |
Molecular weight |
35.8 |
Isoelectric point (calc.) |
8.2 |
Signal SEQ |
|
No. of transmembrane domain |
2 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLKEFEVALLL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |