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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC4G3.11
Gene ID 13/D04
Gene synonyms/obsolete
Gene product conserved hypothetical protein
Entry clone Cloned
ORF length (unspliced) 951
ORF length (spliced)
Entry clone length 951
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC4G3.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGAAAGTTAATCCGTCG
Rev primer name SPCC4G3.11.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAGCGAGAACTACCATCA
Amino acid length 316
Molecular weight 37.1
Isoelectric point (calc.) 10.3
Signal SEQ
No. of transmembrane domain 4
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LTSKIINLPI
Localization (YFP) ER with discontinuity
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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