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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC4A8.09c
Gene ID 13/D08
Gene synonyms/obsolete cwf21
Gene product involved in mRNA splicing; spliceosome component; complexed with Cdc5p; RNA-binding protein (inferred from context); no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 953
ORF length (spliced) 882
Entry clone length 953
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC4A8.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTAAGAGATGCATAAAA
Rev primer name SPAC4A8.09.Rv
Rev primer SEQ AGAAAGCTGGGTATACAATTTCGCCACCCTCA
Amino acid length 293
Molecular weight 34.5
Isoelectric point (calc.) 8.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) SPB?; nuclear dots; nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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