Gene name |
SPBC30B4.07c |
Gene ID |
13/E03 |
Gene synonyms/obsolete |
|
Gene product |
transcription
initiation factor TFIIH subunit; involved in transcription
initiation; involved in DNA repair; involved in
nucleotide-excision repair |
Entry clone |
Cloned |
ORF length (unspliced) |
956 |
ORF length (spliced) |
894 |
Entry clone length |
956 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
449A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC30B4.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATGAATTTCAAACTTT |
Rev primer name |
SPBC30B4.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGTGTGCAATTTTTTTGAC |
Amino acid length |
297 |
Molecular weight |
33.2 |
Isoelectric point (calc.) |
8.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |