Gene name |
SPBC106.16 |
Gene ID |
13/E11 |
Gene synonyms/obsolete |
|
Gene product |
20S proteasome
component (alpha 4); involved in ubiquitin-dependent
proteolysis; proteasome endopeptidase activity |
Entry clone |
Cloned |
ORF length (unspliced) |
958 |
ORF length (spliced) |
780 |
Entry clone length |
958 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
591T:addition |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC106.16.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGGGATATGATCGTGC |
Rev primer name |
SPBC106.16.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTGAATCGTAGAAGTTGTA |
Amino acid length |
259 |
Molecular weight |
28.2 |
Isoelectric point (calc.) |
7.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |