Gene name |
SPAC15A10.05c |
Gene ID |
13/F06 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; hypothetical protein; YjeF domain |
Entry clone |
Cloned |
ORF length (unspliced) |
962 |
ORF length (spliced) |
729 |
Entry clone length |
962 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC15A10.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTATTTCGGTAAGCAA |
Rev primer name |
SPAC15A10.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACCATTGAGAGGGGCTTG |
Amino acid length |
242 |
Molecular weight |
26.4 |
Isoelectric point (calc.) |
7.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LCKQLDDLDI |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |