| Gene name |
SPAC26F1.07 |
| Gene ID |
13/G02 |
| Gene synonyms/obsolete |
|
| Gene product |
aldo/keto reductase;
2-methylbutyraldehyde reductase; similar to Sp SPBC8E4.04
(paralog); putative NADPH co-factor; involved in isoleucine
catabolism; involved in stress response |
| Entry clone |
Cloned |
| ORF length (unspliced) |
966 |
| ORF length (spliced) |
|
| Entry clone length |
966 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC26F1.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGCTGAACAAAAGTA |
| Rev primer name |
SPAC26F1.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGTACGACCGTCTTCCAAG |
| Amino acid length |
321 |
| Molecular weight |
36.1 |
| Isoelectric point (calc.) |
8.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEKTLKDLKL |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |