Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC13G6.09
Gene ID 13/G11
Gene synonyms/obsolete
Gene product similar to C. elegans R07E5.10 and human and R. norvegicus RP-8 proteins; programmed cell death protein 2 C-terminal domain; zinc finger protein; zf-MYND type (inferred from context); non-essential; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 970
ORF length (spliced) 825
Entry clone length 970
No. of intron 1
Sequence status Finished
Sequence results 769T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC13G6.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAGATGTTGATTTGGG
Rev primer name SPAC13G6.09.Rv
Rev primer SEQ AGAAAGCTGGGTATTCAGGTGCTATAATTCCT
Amino acid length 274
Molecular weight 31
Isoelectric point (calc.) 4.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.