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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC57A10.10c
Gene ID 13/H03
Gene synonyms/obsolete sla1
Gene product La protein homolog; RNA-binding protein; phosphoprotein; non-essential; involved in tRNA maturation; rrm RNA recognition motif
Entry clone Cloned
ORF length (unspliced) 972
ORF length (spliced) 897
Entry clone length 972
No. of intron 1
Sequence status Finished
Sequence results 15A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC57A10.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAACTGAAGAACAGAA
Rev primer name SPAC57A10.10.Rv
Rev primer SEQ AGAAAGCTGGGTACTCCGAAGCGGCCGAAGGC
Amino acid length 298
Molecular weight 34.6
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 254
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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