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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC2F7.02c
Gene ID 14/A05
Gene synonyms/obsolete
Gene product protein phosphatase; involved in sodium stress response; NLI interacting factor family
Entry clone Cloned
ORF length (unspliced) 978
ORF length (spliced)
Entry clone length 978
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC2F7.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAATCAACGAAAACCCA
Rev primer name SPAC2F7.02.Rv
Rev primer SEQ AGAAAGCTGGGTATAACTGAAGATTAAGGACA
Amino acid length 325
Molecular weight 36.6
Isoelectric point (calc.) 5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) periphery
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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