Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPCC965.05c
Gene ID 14/A10
Gene synonyms/obsolete thp1
Gene product uracil DNA N-glycosylase activity; involved in DNA repair; involved in base-excision repair; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 978
ORF length (spliced)
Entry clone length 978
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC965.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAACGACATTGAGACGAG
Rev primer name SPCC965.05.Rv
Rev primer SEQ AGAAAGCTGGGTAGACTGCATGTTTCACTATT
Amino acid length 325
Molecular weight 36.5
Isoelectric point (calc.) 9.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 26
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.