Gene name |
SPCC965.05c |
Gene ID |
14/A10 |
Gene synonyms/obsolete |
thp1 |
Gene product |
uracil DNA
N-glycosylase activity; involved in DNA repair; involved in
base-excision repair; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
978 |
ORF length (spliced) |
|
Entry clone length |
978 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC965.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACGACATTGAGACGAG |
Rev primer name |
SPCC965.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGACTGCATGTTTCACTATT |
Amino acid length |
325 |
Molecular weight |
36.5 |
Isoelectric point (calc.) |
9.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
26 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |