| Gene name |
SPAC1851.03 |
| Gene ID |
14/B01 |
| Gene synonyms/obsolete |
ckb1 |
| Gene product |
casein kinase II (beta
subunit) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
980 |
| ORF length (spliced) |
696 |
| Entry clone length |
980 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
11A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1851.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAGCTATACTCTTCGGA |
| Rev primer name |
SPAC1851.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGTTATTGCCAGTTTATTT |
| Amino acid length |
231 |
| Molecular weight |
26.6 |
| Isoelectric point (calc.) |
6.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB; nucleus |
| Comments for localization |
one nuclear dot by
over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |