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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3F10.05c
Gene ID 14/B05
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 981
ORF length (spliced)
Entry clone length 981
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3F10.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAATGCCGAATACTCT
Rev primer name SPAC3F10.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCATAAATGACCCGAGAA
Amino acid length 326
Molecular weight 37.8
Isoelectric point (calc.) 7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) periphery at cell tip and site of septum formation
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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