Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1718.05
Gene ID 14/B07
Gene synonyms/obsolete trs31
Gene product TRAPP plays a key role in the late stages of endoplasmic reticulum to Golgi traffic; Similarity Belongs to the TRAPP small subunits family; BET3 subfamily.
Entry clone Cloned
ORF length (unspliced) 981
ORF length (spliced) 630
Entry clone length 981
No. of intron 3
Sequence status Finished
Sequence results 83T:C / 518T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1718.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAATCCACTAATAAAGA
Rev primer name SPBC1718.05.Rv
Rev primer SEQ AGAAAGCTGGGTATCCCAAAACTTCCTCTCTA
Amino acid length 209
Molecular weight 23.8
Isoelectric point (calc.) 8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>=cytosol; nuclear envelope?
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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