Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPBC13E7.09
Gene ID 14/B09
Gene synonyms/obsolete
Gene product verprolin; proline-rich protein; involved in actin cytoskeletal organization; actin cortical patch component; Wiskott-Aldrich homolog; involved in endocytosis
Entry clone Cloned
ORF length (unspliced) 982
ORF length (spliced) 930
Entry clone length 982
No. of intron 1
Sequence status Finished
Sequence results 11C:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC13E7.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCACCTGCACCACCGCC
Rev primer name SPBC13E7.09.Rv
Rev primer SEQ AGAAAGCTGGGTAAAATGAAGAGAGATTCAAA
Amino acid length 309
Molecular weight 31.2
Isoelectric point (calc.) 11.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.