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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC16A11.10c
Gene ID 14/B11
Gene synonyms/obsolete oca8
Gene product cytochrome b5; electron transporter; overexpression results in cell cycle defects; similar to Sp SPBC29A10.16C
Entry clone Cloned
ORF length (unspliced) 982
ORF length (spliced) 390
Entry clone length 982
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC16A11.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCAGAGAAGACAATTAC
Rev primer name SPCC16A11.10.Rv
Rev primer SEQ AGAAAGCTGGGTATTTGAGGACATACTTGCGA
Amino acid length 129
Molecular weight 14.2
Isoelectric point (calc.) 4.5
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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