Gene name |
SPAC18B11.06 |
Gene ID |
14/C02 |
Gene synonyms/obsolete |
|
Gene product |
U3 snoRNP component;
involved in rRNA processing |
Entry clone |
Cloned |
ORF length (unspliced) |
984 |
ORF length (spliced) |
|
Entry clone length |
984 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC18B11.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATGTATTAAATAAGAG |
Rev primer name |
SPAC18B11.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACGACGTTTCAAATTCTTC |
Amino acid length |
327 |
Molecular weight |
37.6 |
Isoelectric point (calc.) |
9.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
317 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVSLKCQLLL |
Localization (YFP) |
one nuclear dot |
Comments for localization |
nucleolus?; 1~2 dots
on nucleus |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |