| Gene name |
SPBC18H10.10c |
| Gene ID |
14/C08 |
| Gene synonyms/obsolete |
cwc16 |
| Gene product |
eukaryotic conserved
protein; interacts physically with cwf3p (2-hybrid S.
cerevisiae); involved in mRNA splicing; complexed with
Cdc5p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
985 |
| ORF length (spliced) |
885 |
| Entry clone length |
985 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC18H10.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGGAAATATATTCCCCC |
| Rev primer name |
SPBC18H10.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATCCTTCATCATCACTCACT |
| Amino acid length |
294 |
| Molecular weight |
34.1 |
| Isoelectric point (calc.) |
9.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |