Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC664.01c
Gene ID 14/C10
Gene synonyms/obsolete swi6; SPAC824.10c
Gene product involved in mating-type switching (required); silent heterochromatin component; involved in chromosome segregation (required); involved in chromosome cohesion (required); involved in centromere function; involved in the association of Rad26p with centromeres (required); deletion mutant results in lagging chromosomes at mitosis at reduced temperature (frequent); chromodomain protein; binds centromeric flanking sequence; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 987
ORF length (spliced)
Entry clone length 987
No. of intron 0
Sequence status Finished
Sequence results 851A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC664.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGAAAGGAGGTGTTCG
Rev primer name SPAC664.01.Rv
Rev primer SEQ AGAAAGCTGGGTATTCATTTTCACGGAACGTT
Amino acid length 328
Molecular weight 37.2
Isoelectric point (calc.) 4.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 150
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) SPB; nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.