Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1805.06c
Gene ID 14/D03
Gene synonyms/obsolete hem2
Gene product delta-aminolevulinic acid dehydratase
Entry clone Cloned
ORF length (unspliced) 990
ORF length (spliced)
Entry clone length 990
No. of intron 0
Sequence status Finished
Sequence results 109T:C / 500C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1805.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTGTTGATATCAGTTC
Rev primer name SPAC1805.06.Rv
Rev primer SEQ AGAAAGCTGGGTAATATTCTAGCCATTCCAAT
Amino acid length 329
Molecular weight 36.1
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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