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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC11H11.02c
Gene ID 14/D10
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 991
ORF length (spliced) 795
Entry clone length 991
No. of intron 3
Sequence status Finished
Sequence results 593A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC11H11.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATGATGAAGTCTATAA
Rev primer name SPAC11H11.02.Rv
Rev primer SEQ AGAAAGCTGGGTACCAGTAATTTCGATAATCA
Amino acid length 264
Molecular weight 30.6
Isoelectric point (calc.) 8.1
Signal SEQ
No. of transmembrane domain 6
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LTALNNLRI/LWSFSPSLWI/LHFILNVLLFL/LAGLSVLLL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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