| Gene name |
SPAC4G9.13c |
| Gene ID |
14/D12 |
| Gene synonyms/obsolete |
vps26; pep8 |
| Gene product |
retromer complex;
involved in intracellular protein transport; involved in
retrograde vesicle formation from the late vacuole |
| Entry clone |
Cloned |
| ORF length (unspliced) |
993 |
| ORF length (spliced) |
897 |
| Entry clone length |
993 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
(+1)T:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC4G9.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTACTTCTTTAAAAG |
| Rev primer name |
SPAC4G9.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTCATGCTCATCCCTTCTT |
| Amino acid length |
298 |
| Molecular weight |
35.7 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |