| Gene name |
SPAC6F6.10c |
| Gene ID |
14/E05 |
| Gene synonyms/obsolete |
|
| Gene product |
ARP2/3
actin-organizing complex; involved in actin cortical patch
assembly; involved in actin assembly and function; involved in
endocytosis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
994 |
| ORF length (spliced) |
954 |
| Entry clone length |
994 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC6F6.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTCTCATTAGATTATAA |
| Rev primer name |
SPAC6F6.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCACGAACAAATGACCTA |
| Amino acid length |
317 |
| Molecular weight |
36.9 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytoplasmic dots at
cell tip and site of septum formation;
nucleus>=cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol; cytoplasmic dots at cell tip and site of
septum formation |
| Microscope used for
observation |
DeltaVision |