Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC13E7.06
Gene ID 14/E09
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan; predicted coiled-coil region
Entry clone Cloned
ORF length (unspliced) 996
ORF length (spliced)
Entry clone length 996
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC13E7.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACCACCCCCATTGTTTT
Rev primer name SPBC13E7.06.Rv
Rev primer SEQ AGAAAGCTGGGTAAAATTTGTCGTGCATATTT
Amino acid length 331
Molecular weight 38.4
Isoelectric point (calc.) 7.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots; vacuole membrane?
Comments for localization Golgi?
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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