Gene name |
SPCC306.06c |
Gene ID |
14/F02 |
Gene synonyms/obsolete |
|
Gene product |
possibly involved in
cell wall maintenance; possibly involved in maintaining
beta,-1,6-glucan levels |
Entry clone |
Cloned |
ORF length (unspliced) |
998 |
ORF length (spliced) |
936 |
Entry clone length |
998 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
121T:C / 318A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC306.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTTCTTGGAGTATTTT |
Rev primer name |
SPCC306.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAACTGTTTGCGTCGAGGA |
Amino acid length |
311 |
Molecular weight |
34.6 |
Isoelectric point (calc.) |
4.6 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAVLVPTLFI |
Localization (YFP) |
ER; vacuole
membrane? |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |