| Gene name |
SPCC1020.07 |
| Gene ID |
14/G07 |
| Gene synonyms/obsolete |
|
| Gene product |
haloacid
dehalogenase-like hydrolase; similar to Sp SPAC4C5.01
(paralog) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1004 |
| ORF length (spliced) |
711 |
| Entry clone length |
1004 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1020.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTCCTGAGGCGTGTCT |
| Rev primer name |
SPCC1020.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATACCTTTGTAACATCGAAA |
| Amino acid length |
236 |
| Molecular weight |
26.4 |
| Isoelectric point (calc.) |
6.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLSKLKSLNI |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |