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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC553.06
Gene ID 14/H01
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 1006
ORF length (spliced) 816
Entry clone length 1006
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC553.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAGGATTTTTTTTAAT
Rev primer name SPCC553.06.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGACTTTTAGCAGCGATT
Amino acid length 271
Molecular weight 29.3
Isoelectric point (calc.) 5.5
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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