Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC806.01
Gene ID 15/A02
Gene synonyms/obsolete SPAC24B11.13
Gene product porphobilinogen deaminase (putative)
Entry clone Cloned
ORF length (unspliced) 1011
ORF length (spliced)
Entry clone length 1011
No. of intron 0
Sequence status Finished
Sequence results 805T:C / 941A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC806.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCATCCTGTACTTCATT
Rev primer name SPAC806.01.Rv
Rev primer SEQ AGAAAGCTGGGTAATAATTTTTTAAAGATTCT
Amino acid length 336
Molecular weight 36.8
Isoelectric point (calc.) 6.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots
Comments for localization cytoplasmic dots near cell tip and site of septum formation
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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