Gene name |
SPBC16D10.01c |
Gene ID |
15/A06 |
Gene synonyms/obsolete |
SPBC418.03c |
Gene product |
TPR repeat protein;
possibly interacts with protein phosphatase 2C |
Entry clone |
Cloned |
ORF length (unspliced) |
1011 |
ORF length (spliced) |
|
Entry clone length |
1011 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
308C:T / 361A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC16D10.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGTAACCGACGCCGA |
Rev primer name |
SPBC16D10.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCTTCATCCATTTCCTCT |
Amino acid length |
336 |
Molecular weight |
38.1 |
Isoelectric point (calc.) |
4.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |