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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC14C4.06c
Gene ID 15/A09
Gene synonyms/obsolete
Gene product zinc finger protein (inferred); involved in nuclear export; involved in poly(A)+ mRNA-nucleus export; RNA-binding protein; poly(A) binding
Entry clone Cloned#
ORF length (unspliced) 1013
ORF length (spliced) 924
Entry clone length 1013
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC14C4.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTACATTACTGGAAAC
Rev primer name SPAC14C4.06.Rv
Rev primer SEQ AGAAAGCTGGGTACACAGAAGGAACATGAAGT
Amino acid length 307
Molecular weight 33.9
Isoelectric point (calc.) 5.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots; nuclear envelope?; nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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