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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC8E11.05c
Gene ID 15/B03
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 1017
ORF length (spliced)
Entry clone length 1017
No. of intron 0
Sequence status Finished
Sequence results 267T:C / 278A:G / 345A:T / 811T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC8E11.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCAAGTCCGCCAATATT
Rev primer name SPAC8E11.05.Rv
Rev primer SEQ AGAAAGCTGGGTATTTATGAGAGGAAGTGCTC
Amino acid length 338
Molecular weight 38.2
Isoelectric point (calc.) 4.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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