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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC13B11.03c
Gene ID 15/C05
Gene synonyms/obsolete
Gene product hydroxyacylglutathione hydrolase; glyoxalase II family; similar to Sp SPAC824.07
Entry clone Cloned
ORF length (unspliced) 1020
ORF length (spliced) 771
Entry clone length 1020
No. of intron 2
Sequence status Finished
Sequence results 189A:G / 368A:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC13B11.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTTTCAAATATTACC
Rev primer name SPCC13B11.03.Rv
Rev primer SEQ AGAAAGCTGGGTATCCTTGGTTCTTCAACGTG
Amino acid length 256
Molecular weight 28.6
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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