Gene name |
SPAC1D4.13 |
Gene ID |
15/C10 |
Gene synonyms/obsolete |
ste1; byr1; ste3 |
Gene product |
serine/threonine
protein kinase; MAP kinase kinase (MAPKK); involved in
conjugation; involved in sporulation; pheromone signalling
pathway |
Entry clone |
Cloned# |
ORF length (unspliced) |
1023 |
ORF length (spliced) |
|
Entry clone length |
1023 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1D4.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTAAACGACGTCGAAA |
Rev primer name |
SPAC1D4.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACGAGGATCTAAAGTTGGAG |
Amino acid length |
340 |
Molecular weight |
38.1 |
Isoelectric point (calc.) |
7.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIYLYNVLHI/LPSSFPEDLRL |
Localization (YFP) |
nucleus>=cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |