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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP4H10.17c
Gene ID 15/D10
Gene synonyms/obsolete
Gene product carboxyl methyl esterase
Entry clone Cloned
ORF length (unspliced) 1026
ORF length (spliced)
Entry clone length 1026
No. of intron 0
Sequence status Finished
Sequence results 17A:T / 47A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP4H10.17.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCATTTCGTAAAGAAGA
Rev primer name SPBP4H10.17.Rv
Rev primer SEQ AGAAAGCTGGGTACTGTAGAACAGGAGTAGCA
Amino acid length 341
Molecular weight 38
Isoelectric point (calc.) 4.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LNGTINGYLTL
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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