| Gene name |
SPAC637.06 |
| Gene ID |
15/G11 |
| Gene synonyms/obsolete |
|
| Gene product |
galactosyltransferase;
alpha-1,2-galactosyltransferase; GMA12/MNN10 family; predicted
N-terminal signal sequence; involved in galactosylation of
cell surface glycoproteins; similar to Sp gmh1 and gmh3 and
gmh2 and gma12 and SPAC5H10.13C and SPBC1289.13c and
SPBC8D2.17 (paralogs) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1044 |
| ORF length (spliced) |
|
| Entry clone length |
1044 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC637.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTGGAACCCCAAAAAGAA |
| Rev primer name |
SPAC637.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGAAACGGAACTAACTTC |
| Amino acid length |
347 |
| Molecular weight |
41.1 |
| Isoelectric point (calc.) |
9.1 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFVFVVLLL |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |