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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC16A3.02c
Gene ID 15/H01
Gene synonyms/obsolete
Gene product quinone oxidoreductase; zinc binding dehydrogenase
Entry clone Cloned
ORF length (unspliced) 1044
ORF length (spliced)
Entry clone length 1044
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC16A3.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTAGCGAAACCAAAAG
Rev primer name SPBC16A3.02.Rv
Rev primer SEQ AGAAAGCTGGGTAATCCGTCTTAATAATAACT
Amino acid length 347
Molecular weight 38.1
Isoelectric point (calc.) 7.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) Golgi?; ER; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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