Gene name |
SPCC4F11.02 |
Gene ID |
15/H03 |
Gene synonyms/obsolete |
ptc1 |
Gene product |
experimentally
characterised; protein phosphatase 2C; involved in heat shock
response |
Entry clone |
Cloned |
ORF length (unspliced) |
1044 |
ORF length (spliced) |
|
Entry clone length |
1044 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
645T:C / 736T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC4F11.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGGGAAGCCATCCAAA |
Rev primer name |
SPCC4F11.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATAATAGTCATTACTGTAT |
Amino acid length |
347 |
Molecular weight |
38.6 |
Isoelectric point (calc.) |
6.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |