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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC285.05
Gene ID 16/A02
Gene synonyms/obsolete
Gene product conserved protein; involved in purine transport; no apparent Sc ortholog
Entry clone Cloned#
ORF length (unspliced) 1047
ORF length (spliced)
Entry clone length 1047
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC285.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTGTTCTTAAAACTTGT
Rev primer name SPCC285.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAGTCCAGTAAGACCAATGA
Amino acid length 348
Molecular weight 38.1
Isoelectric point (calc.) 4.5
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKLVASVLAL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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