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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC22E12.18
Gene ID 16/A06
Gene synonyms/obsolete
Gene product conserved hypothetical protein
Entry clone Cloned
ORF length (unspliced) 1050
ORF length (spliced) 1011
Entry clone length 1050
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC22E12.18.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGACACTATGGTAAA
Rev primer name SPAC22E12.18.Rv
Rev primer SEQ AGAAAGCTGGGTATTTTTGAGATACTATTTCC
Amino acid length 336
Molecular weight 38.4
Isoelectric point (calc.) 4.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 250
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization changed to: cytosol=nucleus
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images
LMB 4 images) See all images

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