| Gene name |
SPCC11E10.03 |
| Gene ID |
16/B10 |
| Gene synonyms/obsolete |
|
| Gene product |
predicted coiled-coil
region; involved in nuclear migration (sensu Fungi); involved
in control of astral microtubules during mitotic growth |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1056 |
| ORF length (spliced) |
|
| Entry clone length |
1056 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
199A:T / 659T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC11E10.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAGAAATGATTAATCT |
| Rev primer name |
SPCC11E10.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTGCAATTTTAAAACGCGT |
| Amino acid length |
351 |
| Molecular weight |
41.2 |
| Isoelectric point (calc.) |
4.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNKCMEKLEI |
| Localization (YFP) |
cytoplasmic dots;
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |