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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC27.02c
Gene ID 16/B11
Gene synonyms/obsolete
Gene product DASH complex; microtubule-binding complex; involved in chromosome segregation; phosphoprotein; non-essential
Entry clone Cloned#
ORF length (unspliced) 1057
ORF length (spliced) 924
Entry clone length 1057
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC27.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAACAATTTGGAACAGTA
Rev primer name SPBC27.02.Rv
Rev primer SEQ AGAAAGCTGGGTAGAGTTCCCAACTATCTTCT
Amino acid length 307
Molecular weight 34.5
Isoelectric point (calc.) 4.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) SPB; nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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