| Gene name |
SPBC25B2.06c |
| Gene ID |
16/B12 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved hypothetical
protein; POZ domain |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
1057 |
| ORF length (spliced) |
855 |
| Entry clone length |
1057 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC25B2.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAATGAACAGCTCAAC |
| Rev primer name |
SPBC25B2.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATCTAACAGAAGTCGCCTTC |
| Amino acid length |
284 |
| Molecular weight |
31.7 |
| Isoelectric point (calc.) |
6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
bright nuclear dots
and cytoplasmic dots by over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |