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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23H4.12
Gene ID 16/C09
Gene synonyms/obsolete alp13
Gene product chromodomain protein; MRG family; involved in cell polarity (required); histone acetyltransferase complex; involved in histone deacetylation; involved in chromatin silencing; involved in chromatin assembly/disassembly
Entry clone Cloned
ORF length (unspliced) 1063
ORF length (spliced) 1014
Entry clone length 1063
No. of intron 1
Sequence status Finished
Sequence results 667A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23H4.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCCGTGTCATATAAGGT
Rev primer name SPAC23H4.12.Rv
Rev primer SEQ AGAAAGCTGGGTATACACCAACCAAAGATCTA
Amino acid length 337
Molecular weight 39.1
Isoelectric point (calc.) 5.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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