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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC30D11.07
Gene ID 16/D03
Gene synonyms/obsolete nth1
Gene product DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) lyase activity; involved in DNA repair; involved in nucleotide-excision repair; involved in base-excision repair; deletion mutant sensitive to MMS
Entry clone Cloned
ORF length (unspliced) 1068
ORF length (spliced)
Entry clone length 1068
No. of intron 0
Sequence status Finished
Sequence results 272A:G / 451G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC30D11.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTAAAGACTACGGAAC
Rev primer name SPAC30D11.07.Rv
Rev primer SEQ AGAAAGCTGGGTAAACCAGATCTTCAATATCT
Amino acid length 355
Molecular weight 40.2
Isoelectric point (calc.) 8.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKEKLAGGLCL
Localization (YFP) nucleus; nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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