Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC24B10.10c
Gene ID 16/D08
Gene synonyms/obsolete
Gene product AAA family ATPase; involved in intra-mitochondrial sorting
Entry clone Cloned
ORF length (unspliced) 1068
ORF length (spliced)
Entry clone length 1068
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC24B10.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATCCGACGACTAAAAG
Rev primer name SPCC24B10.10.Rv
Rev primer SEQ AGAAAGCTGGGTACTCGACGTCTATACCGGAA
Amino acid length 355
Molecular weight 10
Isoelectric point (calc.) 6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization aggregates
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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