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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1687.13c
Gene ID 16/E01
Gene synonyms/obsolete csn5
Gene product COP9/signalosome complex (subunit 5); MOV34 domain; involved in deneddylation
Entry clone Cloned
ORF length (unspliced) 1071
ORF length (spliced) 900
Entry clone length 1071
No. of intron 2
Sequence status Finished
Sequence results 67T:C / 1018T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1687.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATAATCAACTCGAAAA
Rev primer name SPAC1687.13.Rv
Rev primer SEQ AGAAAGCTGGGTATTCTTTAAATAATTTATCT
Amino acid length 299
Molecular weight 34.3
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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