Gene name |
SPCC285.17 |
Gene ID |
16/F11 |
Gene synonyms/obsolete |
|
Gene product |
upstream activation
factor complex; involved in the activation of RNA polymerase
I; SWIB domain |
Entry clone |
Cloned# |
ORF length (unspliced) |
1078 |
ORF length (spliced) |
702 |
Entry clone length |
1078 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC285.17.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAGAGTACGAAACAGA |
Rev primer name |
SPCC285.17.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCGGTGGATTCGGCGGTA |
Amino acid length |
233 |
Molecular weight |
26.6 |
Isoelectric point (calc.) |
9.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
93 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSPKLAEFLGL |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |